specification sheet
product name: mycoplasma pneumoniae, grade 2
catalog #: ba-na008
description: the resulting antigen preparation contains soluble mycoplasma component in pbs.
inactivation: ether inactivation
cultured in: broth base medium
strain: fh
buffer: pbs
applications: widely used for both igg and igm detection in assays which include eia with polystyrene and latex solid phases.
storage: store at -70℃ to -100℃. repeated freezing and thawing should be avoided.
hazards: inactivated and should contain no infectious material. generally accepted good laboratory practices appropriate to biological should be employed when handling this product.
recommendations for use: this antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. this preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format.
quality control information
product name: mycoplasma pneumoniae, grade 2
bioar performs quality control tests to ensure each batch meets in-horse specification. it is important that each user performance demands on the antigen.
test:
titre: this antigen preparation is titrated using a microtitre plate based elisa. antigens are tested for reactivity with igg. the dilution of antigen which generates a singal of 1.0 o.d unit in the immunoassay is compared to that the standard approved antigen. the result of this comparison is expressed as a percentage of the reference.
igg result: 154.5%
protein concentration: protein is determined using the dye binding assay in the microassay format. the standard curve is generated with a known concentration of igg.
rusult: 2.19 mg/ml
inativation assay: the effectiveness of inactivation is determined when no detection of growth in two blind passages under original culture condition.
result: no growth detected

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