camp [26002-1]
[30 µl]
catalog number: 26002-1
gene symbol:
description: anti-camp mouse monoclonal antibody
background: camp is a ubiquitous second messenger mediating cellular responses to various exogenous and endogenous signaling molecules. camp regulates physiological processes by activating protein kinases, gating specific ion channels, modulating cellular cyclic nucleotide concentrations through phosphodiesterases, and activating epac (exchange protein directly activated by camp). the conversion of atp to camp is catalyzed by adenylyl cyclases (acs). the major family of acs in mammals is the transmembrane acs which have nine isoforms and could be activated by g protein gs and/or ca2+/calmodulin. there is also one soluble ac which could be modulated by bicarbonate and/or ca2+.
immunogen: camp
applications: elisa, wb, ihc
recommended dilutions: elisa 1:1000-1:5000, wb 1:1000-1:2000
host: mouse
format: liquid
clonality: monoclonal
isotype: igg
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg2+ and ca2+), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity:
storage conditions: store at -20°c. avoid freeze / thaw cycles
typical standard curves
sensitivity
acetylated versionmean od for bo = 0.565 ± 0.007mean od for standard #6 = 0.500 ±0.006delta optical density (0-0.08 pmol/ml) = 0.0652 sd's of the zero standard = 0.014sensitivity =(0.014/0.065)*0.08 pmol/ml=17 fmol/ml
publications:
1.
pflugers arch. 2012 feb;463(2):257-68
2.
pharmacol rep. 2012;64(2):351-9
3.
neuropharmacology. 2013 apr;67:272-83
4.
antioxid redox signal. 2013 mar 20;18(9):1009-20
5.
pflugers arch. 2012 feb;463(2):257-68
6.
biochemical and biophysical research communications volume 417, issue 1, 6 january 2012, pages 324–329
7.
pflügers archiv - european journal of physiology october 2013, volume 465, issue 10, pp 1477-1486
8.
the journal of physiology volume 591, issue 14, pages 3605–3623, july 2013
9.
annals of neurology volume 75, issue 2, pages 309–316, february 2014
10.
pflügers archiv - european journal of physiology october 2014, volume 466, issue 10, pp 1921-1932
11.
food funct., 2014,5, 2061-2069
12.
febs journal volume 281, issue 21, pages 4792–4804, november 2014
13.
british journal of pharmacology volume 171, issue 23, pages 5361–5375, december 2014
14.
plos one. 2014 oct 24;9(10):e110944
15.
plos one. 2014 feb 27;9(2):e89932
武汉纽斯特生物技术有限公司
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中国 武汉