绿茶代谢物分析
green tea metabolite profiling by lc-ms
conditions
column: ace excel 1.7 c18-amide
dimensions: 100 x 2.1 mm
part number: exl-1712-1002u
mobile phase:
a: 0.01% formic acid in h2o
b: 0.01% formic acid in mecn
time(mins)
%b
0.0
3
2.5
10
8.0
100
8.5
3
10.0
3
flow rate: 0.5 ml/min
detection: exactive accurate mass ms system esi in negative ion mode
analytes between m/z 70-800 monitored
sample: metabolites from green tea extracted into cold water by vortexing for 20mins. samples filtered prior to injection onto column and modular accela lc system

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